Hotspot

trnE-UUC-trnT-GGU

LSC · 54921-55565 · 645 bp

Back to Filipendula

Pi 0.018

MarkerSeek score 73.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 41
pi: 0.018
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAAGGGAGATGTCCTGACCA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	720-763 bp	1.0	87.7
trnE-UUC-trnT-GGU_p2	AAAGGGAGATGTCCTGACCA	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	699-742 bp	1.0	87.5
trnE-UUC-trnT-GGU_p3	AAAGGGAGATGTCCTGACCA	TGGCGTTACTCTACCACTGA	50.0	50.0	58.0	58.1	696-739 bp	1.0	87.3
trnE-UUC-trnT-GGU_p4	GGGAGATGTCCTGACCACTA	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	717-760 bp	1.0	86.8
trnE-UUC-trnT-GGU_p5	AAAGGGAGATGTCCTGACCA	CCGATGACTTACGCCTTACC	50.0	55.0	58.0	58.2	717-760 bp	1.0	86.6