Hotspot

atpB-rbcL

LSC · 50779-51540 · 762 bp

Back to Filipendula

Pi 0.0171

MarkerSeek score 60.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 46
pi: 0.0171
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTTGAGCAATACGCCCCAT	AACACCCGCTTTGAATCCAA	45.0	45.0	57.9	58.3	871-904 bp	1.0	83.0
atpB-rbcL_p2	AGCAATACGCCCCATCTTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	57.9	58.3	866-899 bp	1.0	82.5
atpB-rbcL_p3	AACCCCAGGACCAGAAGTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.3	58.3	830-863 bp	1.0	81.2
atpB-rbcL_p4	GAGCAATACGCCCCATCTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	58.3	58.3	867-900 bp	1.0	81.1
atpB-rbcL_p5	ATTTGAGCAATACGCCCCAT	TAACACCCGCTTTGAATCCA	45.0	45.0	57.9	57.4	872-905 bp	1.0	80.9