Hotspot

rpoB-trnC-GCA

LSC · 26854-28057 · 1204 bp

Back to Filipendula

Pi 0.0166

MarkerSeek score 53.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 74
pi: 0.0166
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCTCATTCCCACCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.4	58.1	1292-1306 bp	1.0	73.3
rpoB-trnC-GCA_p2	ATTTCCTCATTCCCACCCCC	TTTCGTGATTAGGCGACACC	55.0	50.0	59.4	58.3	1311-1325 bp	1.0	71.6
rpoB-trnC-GCA_p3	ATTTCCTCATTCCCACCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	59.4	58.4	1274-1288 bp	1.0	71.2
rpoB-trnC-GCA_p4	TTTCCTCATTCCCACCCCC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.9	58.1	1291-1305 bp	1.0	69.0
rpoB-trnC-GCA_p5	TTTCCTCATTCCCACCCCC	TTTCGTGATTAGGCGACACC	57.9	50.0	58.9	58.3	1310-1324 bp	1.0	67.3