Hotspot

trnS-GCU-trnG-UCC

LSC · 8346-8946 · 601 bp

Back to Filipendula

Pi 0.0182

MarkerSeek score 72.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 43
pi: 0.0182
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GGGTTGTAGAACGAATCGCA	50.0	50.0	58.0	58.3	455-686 bp	1.0	87.0
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GGGTTGTAGAACGAATCGCA	50.0	50.0	58.0	58.3	454-685 bp	1.0	87.0
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GGGTTGTAGAACGAATCGCA	50.0	50.0	58.2	58.3	509-740 bp	1.0	85.4
trnS-GCU-trnG-UCC_p4	ACGAAAACGGAAAGAGAGGG	GGGTTGTAGAACGAATCGCA	50.0	50.0	57.8	58.3	515-746 bp	1.0	85.2
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	GGGTTGTAGAACGAATCGCA	50.0	50.0	58.4	58.3	457-688 bp	1.0	84.1