Hotspot

trnK-UUU-rps16

LSC · 4201-5140 · 940 bp

Back to Filipendula

Pi 0.0212

MarkerSeek score 61.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 75
pi: 0.0212
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1092-1295 bp	0.9	83.8
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	55.0	55.0	58.0	58.0	1186-1389 bp	0.9	83.6
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CTCGACGAGAGGGAAGAGAT	55.0	55.0	58.0	58.0	1188-1391 bp	0.9	83.6
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AGATCTCGACGAGAGGGAAG	55.0	55.0	58.0	58.0	1209-1395 bp	0.8	78.5
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	ATCTCGACGAGAGGGAAGAG	55.0	55.0	58.0	58.0	1207-1393 bp	0.8	78.5