Hotspot

trnL-UAG-ccsA

SSC · 120144-120254 · 111 bp

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Pi 0.0362
MarkerSeek score 64.80
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
111
variable_sites
19
pi
0.0362
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 AACAAGCCGCTATGGTGAAA CAATTGAAACGACCGAAAAGGA 45.0 40.9 58.1 57.8 226-264 bp 1.0 71.7
trnL-UAG-ccsA_p2 ACAAGCCGCTATGGTGAAAT CAATTGAAACGACCGAAAAGGA 45.0 40.9 57.9 57.8 225-263 bp 1.0 71.5
trnL-UAG-ccsA_p3 GTGAAATCGGTAGACACGCT CAATTGAAACGACCGAAAAGGA 50.0 40.9 58.0 57.8 243-250 bp 0.97561 67.8
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG CAATTGAAACGACCGAAAAGGA 50.0 40.9 58.0 57.8 242-249 bp 0.97561 67.8
trnL-UAG-ccsA_p5 TAGACACGCTGCTCTTAGGA CAATTGAAACGACCGAAAAGGA 50.0 40.9 57.9 57.8 233-240 bp 0.97561 67.1