Hotspot

psbE-petL

LSC · 68964-70216 · 1253 bp

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Pi 0.00709
MarkerSeek score 63.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1253
variable_sites
83
pi
0.00709
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.0 56.3 1367-1410 bp 1.0 74.9
psbE-petL_p2 CAACCCGCAATGAATAGGGA AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.9 56.3 1356-1399 bp 1.0 74.7
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1279-1322 bp 1.0 74.1
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1274-1317 bp 1.0 73.8
psbE-petL_p5 ACCAACCCGCAATGAATAGG AGCTGCTAGTAGAAAACCGA 50.0 45.0 58.2 56.3 1358-1401 bp 1.0 73.2