Hotspot

trnE-UUC-trnT-GGU

LSC · 33435-34101 · 667 bp

Back to Ficus

Pi 0.00797
MarkerSeek score 65.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
667
variable_sites
56
pi
0.00797
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 728-814 bp 1.0 88.7
trnE-UUC-trnT-GGU_p2 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 730-816 bp 1.0 88.6
trnE-UUC-trnT-GGU_p3 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 707-793 bp 1.0 88.4
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 709-795 bp 1.0 88.3
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 727-813 bp 1.0 88.1