Hotspot

atpH-atpI

LSC · 14824-16089 · 1266 bp

Pi 0.00507

MarkerSeek score 68.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 64
pi: 0.00507
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1293-1353 bp	1.0	86.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1349-1409 bp	1.0	85.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1294-1354 bp	1.0	85.3
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	ACAAGTGGCATTCAAGCTCT	45.0	45.0	58.0	57.7	1316-1376 bp	1.0	85.3
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.2	57.9	1353-1413 bp	1.0	85.1