Hotspot

trnG-UCC-trnR-UCU

LSC · 10716-11000 · 285 bp

Back to Ficus

Pi 0.0266
MarkerSeek score 69.70
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
285
variable_sites
30
pi
0.0266
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 320-378 bp 1.0 74.2
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 323-381 bp 1.0 73.9
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 326-384 bp 1.0 73.4
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 329-387 bp 1.0 73.2
trnG-UCC-trnR-UCU_p5 ACCAAAGTTTCACGTTCGGT AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.3 56.9 390-448 bp 1.0 71.7