Hotspot

psbK-psbI

LSC · 8364-8824 · 461 bp

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Pi 0.0128
MarkerSeek score 73.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
461
variable_sites
50
pi
0.0128
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GGATTACGTCCCGGATCATT 50.0 50.0 57.9 57.2 365-597 bp 1.0 84.1
psbK-psbI_p2 CTTTTGTTTGGCAAGCTGCT GGATTACGTCCCGGATCATT 45.0 50.0 58.1 57.2 370-602 bp 1.0 84.0
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TCTTCACGTCCAGGATTACG 50.0 50.0 57.9 57.1 377-609 bp 1.0 83.3
psbK-psbI_p4 CTTTTGTTTGGCAAGCTGCT TCTTCACGTCCAGGATTACG 45.0 50.0 58.1 57.1 382-614 bp 1.0 83.2
psbK-psbI_p5 TGGCAAGCTGCTGTAAGTTT GGATTACGTCCCGGATCATT 45.0 50.0 58.3 57.2 362-594 bp 1.0 82.1