Hotspot

rps16-trnQ-UUG

LSC · 6740-7724 · 985 bp

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Pi 0.0134
MarkerSeek score 74.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
985
variable_sites
101
pi
0.0134
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTATCCTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 804-1073 bp 1.0 91.3
rps16-trnQ-UUG_p2 ACGTTGCCTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 788-1057 bp 1.0 90.6
rps16-trnQ-UUG_p3 GTTGCCTTCTACCACATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 786-1055 bp 1.0 90.6
rps16-trnQ-UUG_p4 CACGTTGCCTTCTACCACAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 789-1058 bp 1.0 89.9
rps16-trnQ-UUG_p5 TCGTATCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 806-1075 bp 1.0 89.2