Hotspot

trnK-UUU-rps16

LSC · 4524-5574 · 1051 bp

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Pi 0.0137
MarkerSeek score 69.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1051
variable_sites
110
pi
0.0137
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC GGAACTTCGCCTTAATCACCA 50.0 47.6 57.9 58.3 1176-1219 bp 0.95122 78.4
trnK-UUU-rps16_p2 AGTCGCGGTCTTACAAACTC TGGAACTTCGCCTTAATCACC 50.0 47.6 57.9 58.3 1177-1220 bp 0.95122 78.4
trnK-UUU-rps16_p3 GTCGCGGTCTTACAAACTCT GGAACTTCGCCTTAATCACCA 50.0 47.6 57.9 58.3 1175-1218 bp 0.95122 78.4
trnK-UUU-rps16_p4 GTCGCGGTCTTACAAACTCT TGGAACTTCGCCTTAATCACC 50.0 47.6 57.9 58.3 1176-1219 bp 0.95122 78.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGAACTTCGCCTTAATCACCA 50.0 47.6 58.2 58.3 1092-1135 bp 0.95122 77.9