Hotspot

trnH-GUG-psbA

LSC · 146-518 · 373 bp

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Pi 0.0162
MarkerSeek score 60.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
373
variable_sites
42
pi
0.0162
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.95
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTAGCGGCTGTAGAAGTTC 55.0 55.0 58.1 57.8 398-445 bp 0.853659 83.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CTAGCGGCTGTAGAAGTTCC 55.0 55.0 58.1 57.8 397-444 bp 0.853659 83.4
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCTAGCGGCTGTAGAAGTTC 50.0 55.0 57.9 57.8 412-459 bp 0.853659 83.4
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA CTAGCGGCTGTAGAAGTTCC 50.0 55.0 57.9 57.8 411-458 bp 0.853659 83.4
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CTAGCGGCTGTAGAAGTTCC 50.0 55.0 57.9 57.8 410-457 bp 0.853659 83.4