Hotspot

rps8

LSC · 81359-81757 · 399 bp

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Pi 0

MarkerSeek score 26.70

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AACCAATGGAGTAGGGGTGA	AAAAACTCGGAGGCACGAAT	50.0	45.0	57.9	58.1	527-529 bp	1.0	79.0
rps8_p2	TCAACCAATGGAGTAGGGGT	AAAAACTCGGAGGCACGAAT	50.0	45.0	57.9	58.1	529-531 bp	1.0	79.0
rps8_p3	GGGTGACATTAGACAACCCC	AAAAACTCGGAGGCACGAAT	55.0	45.0	57.9	58.1	513-515 bp	1.0	78.5
rps8_p4	GGTGACATTAGACAACCCCC	AAAAACTCGGAGGCACGAAT	55.0	45.0	57.9	58.1	512-514 bp	1.0	78.5
rps8_p5	CAACCAATGGAGTAGGGGTG	AAAAACTCGGAGGCACGAAT	55.0	45.0	57.9	58.1	528-530 bp	1.0	78.4