Hotspot

ccsA

SSC · 122955-123926 · 972 bp

Back to Ficaria

Pi 0.00103

MarkerSeek score 51.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CCGGACTTCCATTCCATTGT	TTCTGTTCGTAAAGCCCTCC	50.0	50.0	57.9	57.5	1048-1057 bp	1.0	75.3
ccsA_p2	AGTCCGGACTTCCATTCCAT	TTCTGTTCGTAAAGCCCTCC	50.0	50.0	58.4	57.5	1051-1060 bp	1.0	73.0
ccsA_p3	TCCGGACTTCCATTCCATTG	TTCTGTTCGTAAAGCCCTCC	50.0	50.0	57.6	57.5	1049-1058 bp	1.0	73.0
ccsA_p4	GTCCGGACTTCCATTCCATT	TTCTGTTCGTAAAGCCCTCC	50.0	50.0	57.6	57.5	1050-1059 bp	1.0	73.0
ccsA_p5	CCGGACTTCCATTCCATTGT	ATTCTGTTCGTAAAGCCCTCC	50.0	47.6	57.9	58.0	1049-1058 bp	1.0	70.9