Hotspot

atpF

LSC · 11838-13108 · 1271 bp

Back to Ficaria

Pi 0.000787

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1271
variable_sites: 1
pi: 0.000787
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAGGGTTGCCATGAGTCTTG	TCAAATAAAAGAGGGCGAAGT	50.0	38.1	58.1	55.5	1414 bp	1.0	51.4
atpF_p2	GAAGGGTTGCCATGAGTCTT	TCAAATAAAAGAGGGCGAAGT	50.0	38.1	57.8	55.5	1415 bp	1.0	50.4
atpF_p3	AAGGGTTGCCATGAGTCTTG	AAAGAGGGCGAAGTAATACA	50.0	40.0	58.1	54.1	1407 bp	1.0	48.0
atpF_p4	AAGGGTTGCCATGAGTCTTG	AAGAGGGCGAAGTAATACAA	50.0	40.0	58.1	54.1	1406 bp	1.0	48.0
atpF_p5	AAGGGTTGCCATGAGTCTTG	AGAGGGCGAAGTAATACAAA	50.0	40.0	58.1	54.1	1405 bp	1.0	48.0