Hotspot

rpl32-trnL-UAG

SSC · 121939-122764 · 826 bp

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Pi 0.00121
MarkerSeek score 59.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
826
variable_sites
1
pi
0.00121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAACAAGT AGCGTGTCTACCGATTTCAC 40.0 50.0 57.0 58.0 877-880 bp 1.0 72.0
rpl32-trnL-UAG_p2 TGTGCGACAAACAAACAAGT CAGCGTGTCTACCGATTTCA 40.0 50.0 57.0 58.0 878-881 bp 1.0 72.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAACAAGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.0 57.9 887-890 bp 1.0 71.1
rpl32-trnL-UAG_p4 TGTGCGACAAACAAACAAGT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 57.0 57.8 886-889 bp 1.0 70.5
rpl32-trnL-UAG_p5 TGTGCGACAAACAAACAAGT CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.0 57.8 885-888 bp 1.0 70.5