Hotspot

ycf4-cemA

LSC · 62334-63120 · 787 bp

Back to Ficaria

Pi 0.00127

MarkerSeek score 59.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AACAAAAGGCTGCGGAATTG	ATACCCCAGGGCAAAAAGAC	45.0	50.0	57.8	57.8	911-913 bp	1.0	77.0
ycf4-cemA_p2	AATTGAACAAAAGGCTGCGG	ATACCCCAGGGCAAAAAGAC	45.0	50.0	57.8	57.8	916-918 bp	1.0	77.0
ycf4-cemA_p3	GCCGTTCCCTTAACTCGTAC	ATACCCCAGGGCAAAAAGAC	55.0	50.0	58.4	57.8	960-962 bp	1.0	75.3
ycf4-cemA_p4	GAACAAAAGGCTGCGGAATT	ATACCCCAGGGCAAAAAGAC	45.0	50.0	57.6	57.8	912-914 bp	1.0	74.7
ycf4-cemA_p5	AACAAAAGGCTGCGGAATTG	CCCCAGGGCAAAAAGACTAT	45.0	50.0	57.8	57.5	908-910 bp	1.0	74.6