Hotspot

atpH-atpI

LSC · 13794-14926 · 1133 bp

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Pi 0.000883

MarkerSeek score 59.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 1
pi: 0.000883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATTTTCGCAACTTTAGCCGC	50.0	45.0	58.0	58.0	1265 bp	1.0	79.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTCGCAACTTTAGCCGC	45.0	45.0	58.0	58.0	1222 bp	1.0	79.6
atpH-atpI_p3	CGCAATACCTTCTACGGCTT	ATTTTCGCAACTTTAGCCGC	50.0	45.0	58.1	58.0	1310 bp	1.0	79.4
atpH-atpI_p4	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1238 bp	1.0	79.3
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1195 bp	1.0	79.3