Hotspot

trnK-UUU-rps16

LSC · 4385-5231 · 847 bp

Back to Ficaria

Pi 0.00118

MarkerSeek score 59.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 1
pi: 0.00118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGGGCTCAACCTACG	50.0	50.0	58.2	58.1	946-968 bp	1.0	78.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGGGCTCAACCTACG	50.0	50.0	58.2	58.1	945-967 bp	1.0	78.0
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	AAAAAGGGGCTCAACCTACG	50.0	50.0	58.3	58.1	960-982 bp	1.0	77.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGGGCTCAACCTACG	50.0	50.0	58.3	58.1	955-977 bp	1.0	77.1
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	AAAAAGGGGCTCAACCTACG	50.0	50.0	57.5	58.1	932-954 bp	1.0	75.6