Hotspot

matK-trnK-UUU

LSC · 3556-4347 · 792 bp

Back to Ficaria

Pi 0.00253

MarkerSeek score 59.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 2
pi: 0.00253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TAATCGTGAGCAAGCGCATA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.8	58.2	920 bp	1.0	77.1
matK-trnK-UUU_p2	TAATCGTGAGCAAGCGCATA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.8	58.2	919 bp	1.0	77.1
matK-trnK-UUU_p3	ACCCATAATCGTGAGCAAGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.3	58.2	925 bp	1.0	76.2
matK-trnK-UUU_p4	ACCCATAATCGTGAGCAAGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.3	58.2	924 bp	1.0	76.2
matK-trnK-UUU_p5	TAATCGTGAGCAAGCGCATA	TATTTTGGGTGGGGTTGTCC	45.0	50.0	57.8	57.7	1009 bp	1.0	75.9