Hotspot

psbE-petL

LSC · 60875-62042 · 1168 bp

Back to Festuca

Pi 0.0122

MarkerSeek score 61.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 52
pi: 0.0122
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1224-1346 bp	1.0	76.0
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1222-1344 bp	1.0	76.0
psbE-petL_p3	CAGCAAAAGAACGTTCTCCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1227-1349 bp	1.0	75.8
psbE-petL_p4	CAGCAAAAGAACGTTCTCCC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1225-1347 bp	1.0	75.8
psbE-petL_p5	AGCAAAAGAACGTTCTCCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.8	58.5	1226-1348 bp	1.0	75.1