Hotspot

ndhC-trnV-UAC

LSC · 48909-49816 · 908 bp

Back to Festuca

Pi 0.0127

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 39
pi: 0.0127
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	951-1018 bp	1.0	86.4
ndhC-trnV-UAC_p2	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	952-1019 bp	1.0	84.5
ndhC-trnV-UAC_p3	GCCCTGAAATCCAAAATGCC	AGCCCAATGCACAAAAATGC	50.0	45.0	58.0	58.5	1044-1111 bp	1.0	83.0
ndhC-trnV-UAC_p4	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	970-1037 bp	1.0	80.3
ndhC-trnV-UAC_p5	GCCCTGAAATCCAAAATGCC	GAAAGAGCCCAATGCACAAA	50.0	45.0	58.0	57.2	1116 bp	0.058824	35.6