Hotspot

trnF-GAA-ndhJ

LSC · 46656-47239 · 584 bp

Back to Festuca

Pi 0.0138

MarkerSeek score 66.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 46
pi: 0.0138
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	58.1	57.9	670 bp	0.058824	17.3
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.5	57.9	673 bp	0.058824	14.1
trnF-GAA-ndhJ_p3	TCAGTTGGTAGAGCAGAGGA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.4	57.9	698 bp	0.058824	12.9
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.4	57.9	693 bp	0.058824	12.9
trnF-GAA-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	ACGTAAGGACTATATAACCCCCA	50.0	43.5	57.4	57.9	694 bp	0.058824	12.9