Hotspot

psaA-ycf3

LSC · 40286-40974 · 689 bp

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Pi 0.0141

MarkerSeek score 59.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 36
pi: 0.0141
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.0	58.0	875-923 bp	1.0	87.1
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	802-850 bp	1.0	87.0
psaA-ycf3_p3	CCGCCTACATCACCATTCAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.0	58.0	1106-1154 bp	1.0	86.7
psaA-ycf3_p4	GTTATTCCAGATGCTCGCCA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.0	58.0	1169-1217 bp	1.0	86.6
psaA-ycf3_p5	CACCGGTCTGACTGTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	949 bp	0.058824	41.8