Hotspot

trnC-GCA-rpoB

LSC · 17599-18775 · 1177 bp

Back to Festuca

Pi 0.0104

MarkerSeek score 64.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 52
pi: 0.0104
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	957-1266 bp	1.0	86.5
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	964-1273 bp	1.0	85.4
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	971-1280 bp	1.0	84.3
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	972-1281 bp	1.0	84.2
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	GGACATTCCCTCGTTTCCAT	50.0	50.0	58.1	57.6	961-1270 bp	1.0	83.5