primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 5601 6366 705-859 1.000000 87.4 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 0.0 16.9 0.280333 5601 6366 716-870 1.000000 86.8 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 20 20 45.0 55.0 58.1 57.8 8.1 16.9 0.350677 5601 6366 709-863 1.000000 86.3 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 20 20 50.0 55.0 58.2 57.8 0.0 16.9 0.415585 5601 6366 720-874 1.000000 85.7 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 20 20 45.0 50.0 58.1 57.6 8.1 16.1 0.571278 5601 6366 711-865 1.000000 84.5 psbK-psbI_p1 psbK-psbI 1 GTTTGGCAAGCTGCTGTAAG TTCCGTCCTGGGTCATTAGA 20 20 50.0 50.0 57.9 57.8 1.4 0.0 0.297375 6968 7377 508-538 1.000000 84.8 psbK-psbI_p2 psbK-psbI 2 GTTTGGCAAGCTGCTGTAAG GAATTTTTACTCCTCGCGCC 20 20 50.0 50.0 57.9 57.5 1.4 0.0 0.509744 6968 7377 533-563 1.000000 83.0 psbK-psbI_p3 psbK-psbI 3 GTTTGGCAAGCTGCTGTAAG GGAATTTTTACTCCTCGCGC 20 20 50.0 50.0 57.9 57.5 1.4 0.0 0.509744 6968 7377 534-564 1.000000 83.0 psbK-psbI_p4 psbK-psbI 4 GTTTGGCAAGCTGCTGTAAG TCCGTCCTGGGTCATTAGAT 20 20 50.0 50.0 57.9 57.5 1.4 0.0 0.527909 6968 7377 507-537 1.000000 83.0 psbK-psbI_p5 psbK-psbI 5 TGGCAAGCTGCTGTAAGTTT TTCCGTCCTGGGTCATTAGA 20 20 45.0 50.0 58.3 57.8 1.4 0.0 0.550084 6968 7377 522-535 0.941176 79.0 trnG-UCC-trnT-GGU_p1 trnG-UCC-trnT-GGU 1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 21 20 38.1 45.0 55.5 56.6 0.0 0.0 4.907539 13565 14225 686-1250 1.000000 49.0 trnG-UCC-trnT-GGU_p2 trnG-UCC-trnT-GGU 2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 21 21 38.1 47.6 55.5 57.5 0.0 0.0 5.004851 13565 14225 682-1246 1.000000 48.3 trnG-UCC-trnT-GGU_p3 trnG-UCC-trnT-GGU 3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 21 21 38.1 47.6 55.5 57.5 0.0 0.0 5.006487 13565 14225 683-1247 1.000000 48.3 trnG-UCC-trnT-GGU_p4 trnG-UCC-trnT-GGU 4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 23 20 39.1 45.0 57.3 56.6 0.0 0.0 5.040254 13565 14225 689-1253 1.000000 48.3 trnG-UCC-trnT-GGU_p5 trnG-UCC-trnT-GGU 5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 23 20 39.1 45.0 57.3 56.6 0.0 0.0 5.040254 13565 14225 688-1252 1.000000 48.3 trnD-GUC-psbM_p1 trnD-GUC-psbM 1 TTCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 20 20 50.0 45.0 58.5 55.8 5.4 0.0 2.706630 15453 16149 591-818 1.000000 65.5 trnD-GUC-psbM_p2 trnD-GUC-psbM 2 TAGGTATGCCATACACCCCG AGAATGAACAGTGCAGTAGC 20 20 55.0 45.0 58.7 55.8 16.3 0.0 2.885233 15453 16149 623-850 1.000000 64.0 trnD-GUC-psbM_p3 trnD-GUC-psbM 3 GTTCAATTGGTCAGAGCACC AGAATGAACAGTGCAGTAGC 20 20 50.0 45.0 57.0 55.8 5.4 0.0 3.226784 15453 16149 592-819 1.000000 61.3 trnD-GUC-psbM_p4 trnD-GUC-psbM 4 CGCGGGGATTGTAGTTCAA AGAATGAACAGTGCAGTAGC 19 20 52.6 45.0 57.9 55.8 0.0 0.0 3.376082 15453 16149 605-832 1.000000 60.1 trnD-GUC-psbM_p5 trnD-GUC-psbM 5 TCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 19 20 52.6 45.0 57.8 55.8 5.4 0.0 3.461033 15453 16149 590-817 1.000000 59.5 petN-trnC-GCA_p1 petN-trnC-GCA 1 AGCCCAAGCGAGACTTACTA TTTCTCGATTTTCGCTCGGA 20 20 50.0 45.0 58.2 57.6 0.0 0.0 0.508675 16625 17527 1027-1047 1.000000 80.9 petN-trnC-GCA_p2 petN-trnC-GCA 2 CCACTCCTCCCCCATACTAC TTTCTCGATTTTCGCTCGGA 20 20 60.0 45.0 58.3 57.6 0.0 0.0 0.639047 16625 17527 1083-1103 1.000000 79.7 petN-trnC-GCA_p3 petN-trnC-GCA 3 AGCCCAAGCGAGACTTACTA TCTCGATTTTCGCTCGGATT 20 20 50.0 45.0 58.2 57.4 0.0 0.0 0.730555 16625 17527 1025-1045 1.000000 79.2 petN-trnC-GCA_p4 petN-trnC-GCA 4 AGCCCAAGCGAGACTTACTA TTCTCGATTTTCGCTCGGAT 20 20 50.0 45.0 58.2 57.4 0.0 0.0 0.730555 16625 17527 1026-1046 1.000000 79.1 petN-trnC-GCA_p5 petN-trnC-GCA 5 TCCACTCCTCCCCCATACTA TTTCTCGATTTTCGCTCGGA 20 20 55.0 45.0 58.4 57.6 0.0 0.0 0.729480 16625 17527 1084-1104 1.000000 79.0 trnC-GCA-rpoB_p1 trnC-GCA-rpoB 1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 20 20 50.0 50.0 58.1 58.0 14.6 0.0 0.109519 17599 18775 957-1266 1.000000 86.5 trnC-GCA-rpoB_p2 trnC-GCA-rpoB 2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 20 20 50.0 50.0 58.1 57.8 14.6 0.0 0.250044 17599 18775 964-1273 1.000000 85.4 trnC-GCA-rpoB_p3 trnC-GCA-rpoB 3 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 17599 18775 971-1280 1.000000 84.3 trnC-GCA-rpoB_p4 trnC-GCA-rpoB 4 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 20 20 50.0 55.0 58.1 58.3 14.6 0.0 0.395580 17599 18775 972-1281 1.000000 84.2 trnC-GCA-rpoB_p5 trnC-GCA-rpoB 5 AAAGGATTTGCAGTCCCCTG GGACATTCCCTCGTTTCCAT 20 20 50.0 50.0 58.1 57.6 14.6 0.0 0.491190 17599 18775 961-1270 1.000000 83.5 psaA-ycf3_p1 psaA-ycf3 1 GCATCAGCATGTAGGTTCCA TATAGCGCTTACTCCGGGAA 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.057213 40286 40974 875-923 1.000000 87.1 psaA-ycf3_p2 psaA-ycf3 2 GTCTGGCCCATTCCTCAAAA TATAGCGCTTACTCCGGGAA 20 20 50.0 50.0 58.1 58.0 0.0 18.7 0.091171 40286 40974 802-850 1.000000 87.0 psaA-ycf3_p3 psaA-ycf3 3 CCGCCTACATCACCATTCAA TATAGCGCTTACTCCGGGAA 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.051484 40286 40974 1106-1154 1.000000 86.7 psaA-ycf3_p4 psaA-ycf3 4 GTTATTCCAGATGCTCGCCA TATAGCGCTTACTCCGGGAA 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.060968 40286 40974 1169-1217 1.000000 86.6 psaA-ycf3_p5 psaA-ycf3 5 CACCGGTCTGACTGTCAAAA TATAGCGCTTACTCCGGGAA 20 20 50.0 50.0 58.1 58.0 2.6 18.7 0.066423 40286 40974 949-949 0.058824 41.8 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032006 44916 45606 712-942 1.000000 87.4 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073242 44916 45606 704-934 1.000000 87.1 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 44916 45606 682-912 1.000000 86.6 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 20 20 50.0 55.0 58.0 58.2 0.0 10.2 0.272624 44916 45606 702-932 1.000000 85.5 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 20 20 50.0 50.0 58.0 57.7 0.0 0.0 0.303758 44916 45606 711-941 1.000000 85.2 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 AAGTCGTGAGGGTTCAAGTC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.579937 46242 46582 431-458 1.000000 81.9 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 AAGTCGTGAGGGTTCAAGTC TCTGCCAGGAACCAGATTTG 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.733063 46242 46582 447-474 1.000000 80.7 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 AAGTCGTGAGGGTTCAAGTC CTGCCAGGAACCAGATTTGA 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.733063 46242 46582 446-473 1.000000 80.5 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 AAGTCGTGAGGGTTCAAGTC TTTTCTCTGCCAGGAACCAG 20 20 50.0 50.0 57.5 57.7 0.0 0.0 0.804773 46242 46582 452-479 1.000000 80.4 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 AAGTCGTGAGGGTTCAAGTC TCTCTGCCAGGAACCAGATT 20 20 50.0 50.0 57.5 58.3 0.0 0.0 0.866578 46242 46582 449-476 1.000000 79.9 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 CCTCGTGTCACCAGTTCAAA ACGTAAGGACTATATAACCCCCA 20 23 50.0 43.5 58.1 57.9 0.0 0.0 3.130578 46656 47239 670-670 0.058824 17.3 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 AATCCTCGTGTCACCAGTTC ACGTAAGGACTATATAACCCCCA 20 23 50.0 43.5 57.5 57.9 0.0 0.0 3.536049 46656 47239 673-673 0.058824 14.1 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 TCAGTTGGTAGAGCAGAGGA ACGTAAGGACTATATAACCCCCA 20 23 50.0 43.5 57.4 57.9 0.0 0.0 3.683595 46656 47239 698-698 0.058824 12.9 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 TGGTAGAGCAGAGGACTGAA ACGTAAGGACTATATAACCCCCA 20 23 50.0 43.5 57.4 57.9 0.0 0.0 3.683595 46656 47239 693-693 0.058824 12.9 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 TTGGTAGAGCAGAGGACTGA ACGTAAGGACTATATAACCCCCA 20 23 50.0 43.5 57.4 57.9 0.0 0.0 3.683595 46656 47239 694-694 0.058824 12.9 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.104399 48909 49816 951-1018 1.000000 86.4 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 20 20 50.0 55.0 58.0 58.3 0.0 22.8 0.333780 48909 49816 952-1019 1.000000 84.5 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 GCCCTGAAATCCAAAATGCC AGCCCAATGCACAAAAATGC 20 20 50.0 45.0 58.0 58.5 0.0 0.0 0.502479 48909 49816 1044-1111 1.000000 83.0 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 20 20 50.0 55.0 57.2 57.9 6.3 0.0 0.849972 48909 49816 970-1037 1.000000 80.3 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 GCCCTGAAATCCAAAATGCC GAAAGAGCCCAATGCACAAA 20 20 50.0 45.0 58.0 57.2 0.0 0.0 0.846550 48909 49816 1116-1116 0.058824 35.6 rbcL-psaI_p1 rbcL-psaI 1 GCTTCGGGTGGTATTCATGT CCAAGATAACTGGAAGCCCC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.096980 55006 56182 1755-2116 1.000000 86.9 rbcL-psaI_p2 rbcL-psaI 2 CATAACTGGGGGATTCACCG CCAAGATAACTGGAAGCCCC 20 20 55.0 55.0 58.0 57.9 0.0 0.0 0.091274 55006 56182 2080-2441 1.000000 86.7 rbcL-psaI_p3 rbcL-psaI 3 TTTTTCACTCAGGACTGGGC GTCATTGCAATTGCCGGAAA 20 20 50.0 45.0 58.0 57.9 0.0 26.9 0.096156 55006 56182 1570-1645 0.647059 69.4 rbcL-psaI_p4 rbcL-psaI 4 TTTTTCACTCAGGACTGGGC CCAAGATAACTGGAAGCCCC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.077336 55006 56182 2056-2131 0.647059 69.1 rbcL-psaI_p5 rbcL-psaI 5 TTTTTCACTCAGGACTGGGC CATACGTGTTCTGATCGCCA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.097500 55006 56182 1948-2023 0.647059 69.1 petA-psbJ_p1 petA-psbJ 1 ACGTAAAGAAAAAGGCGGGT CCCATTTGGAAGGATACCCG 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.047262 59478 60104 1153-1305 1.000000 85.0 petA-psbJ_p2 petA-psbJ 2 AAACCGAGGAAGAGGACAGA CCCATTTGGAAGGATACCCG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.029866 59478 60104 1246-1398 1.000000 84.8 petA-psbJ_p3 petA-psbJ 3 AAACCGAGGAAGAGGACAGA TGCGGGTTGGTTATTTGTCA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.028462 59478 60104 1705-1857 1.000000 83.9 petA-psbJ_p4 petA-psbJ 4 ACGTAAAGAAAAAGGCGGGT TGCGGGTTGGTTATTTGTCA 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.045859 59478 60104 1612-1764 1.000000 83.9 petA-psbJ_p5 petA-psbJ 5 CTTGTGATAGGCCCTGTTCC TGCGGGTTGGTTATTTGTCA 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.061463 59478 60104 1824-1976 1.000000 83.4 psbE-petL_p1 psbE-petL 1 CAAAAGAACGTTCTCCCGTG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.139521 60875 62042 1224-1346 1.000000 76.0 psbE-petL_p2 psbE-petL 2 CAAAAGAACGTTCTCCCGTG GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.143870 60875 62042 1222-1344 1.000000 76.0 psbE-petL_p3 psbE-petL 3 CAGCAAAAGAACGTTCTCCC AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.164606 60875 62042 1227-1349 1.000000 75.8 psbE-petL_p4 psbE-petL 4 CAGCAAAAGAACGTTCTCCC GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.168956 60875 62042 1225-1347 1.000000 75.8 psbE-petL_p5 psbE-petL 5 AGCAAAAGAACGTTCTCCCG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 58.8 58.5 29.8 0.0 1.249496 60875 62042 1226-1348 1.000000 75.1 rpl22-rps19_p1 rpl22-rps19 1 CCTAGCGAGTTTCCTTAGCC GGAATTCGTACCTACTCGGC 20 20 55.0 55.0 57.8 57.9 0.0 0.0 0.357883 79011 79088 223-224 1.000000 82.2 rpl22-rps19_p2 rpl22-rps19 2 CTAGCGAGTTTCCTTAGCCC GGAATTCGTACCTACTCGGC 20 20 55.0 55.0 57.8 57.9 0.0 0.0 0.357883 79011 79088 222-223 1.000000 82.2 rpl22-rps19_p3 rpl22-rps19 3 CCTAGCGAGTTTCCTTAGCC ATGGTAGGTCGCAAATTGGG 20 20 55.0 50.0 57.8 58.2 0.0 3.4 0.463894 79011 79088 243-244 1.000000 80.9 rpl22-rps19_p4 rpl22-rps19 4 CTAGCGAGTTTCCTTAGCCC ATGGTAGGTCGCAAATTGGG 20 20 55.0 50.0 57.8 58.2 0.0 3.4 0.463894 79011 79088 242-243 1.000000 80.9 rpl22-rps19_p5 rpl22-rps19 5 CTAGCGAGTTTCCTTAGCCC GTCGCAAATTGGGGGAATTC 20 20 55.0 50.0 57.8 57.7 0.0 10.2 0.520279 79011 79088 235-236 1.000000 80.6 ndhF-rpl32_p1 ndhF-rpl32 1 GGAAGTGGGAGAAGAGGGAT TTCGAACCGAGATGCTTGAG 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.117286 102571 103436 1572-1926 1.000000 89.4 ndhF-rpl32_p2 ndhF-rpl32 2 GAAGAGGGAATTGTGCGGAT TTCGAACCGAGATGCTTGAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.047831 102571 103436 2261-2615 1.000000 88.5 ndhF-rpl32_p3 ndhF-rpl32 3 GAAGAGGGAATTGTGCGGAT GGATTCGAACCGAGATGCTT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.102798 102571 103436 2264-2618 1.000000 88.0 ndhF-rpl32_p4 ndhF-rpl32 4 GAAGAGGGAATTGTGCGGAT GGTTTTGGATGCTCATTGCC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.058458 102571 103436 1656-1814 1.000000 87.2 ndhF-rpl32_p5 ndhF-rpl32 5 GAAGAGGGAATTGTGCGGAT ATGCTCATTGCCGCTAGAAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.086809 102571 103436 1648-1806 0.941176 83.8 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GCAATGAGCATCCAAAACCA TTCGAACCGAGATGCTTGAG 20 20 45.0 50.0 57.3 58.0 0.0 0.0 0.751105 103629 104085 588-837 1.000000 85.9 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AGGGAATAGGCTTCCGTACC TTCGAACCGAGATGCTTGAG 20 20 55.0 50.0 58.6 58.0 0.0 0.0 0.582229 103629 104085 1051-1289 0.941176 83.6 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AGGGAATAGGCTTCCGTACC GGATTCGAACCGAGATGCTT 20 20 55.0 50.0 58.6 58.1 0.0 0.0 0.637195 103629 104085 1054-1292 0.941176 83.1 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AGGGAATAGGCTTCCGTACC TCCTAAGAGCAGCGTGTCTA 20 20 55.0 50.0 58.6 57.9 0.0 0.0 0.702798 103629 104085 1024-1262 0.941176 82.8 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AGGGAATAGGCTTCCGTACC GATCCGCACAAAAGAGTTGC 20 20 55.0 50.0 58.6 58.0 0.0 0.0 0.609417 103629 104085 1603-1841 0.941176 81.2