Hotspot

ycf1

SSC · 124084-129405 · 5322 bp

Back to Farsetia

Pi 0.00939

MarkerSeek score 49.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5322
variable_sites: 50
pi: 0.00939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAACCAATGAAATACCTATGGT	ACTGTCTCTTAGTTAGACTAAAA	34.8	30.4	55.6	52.3	214 bp	1.0	41.9
ycf1_p2	ACCAATGAAATACCTATGGTTCT	AGTATACTGTCTCTTAGTTAGACT	34.8	33.3	55.3	53.5	216 bp	1.0	41.9
ycf1_p3	TGAACCAATGAAATACCTATGGT	ACTGTCTCTTAGTTAGACTAAAAA	34.8	29.2	55.6	53.1	214 bp	1.0	41.9
ycf1_p4	TGAACCAATGAAATACCTATGGT	AGTATACTGTCTCTTAGTTAGACT	34.8	33.3	55.6	53.5	219 bp	1.0	41.8
ycf1_p5	TGAACCAATGAAATACCTATGGT	AAGTATACTGTCTCTTAGTTAGACT	34.8	32.0	55.6	54.3	220 bp	1.0	41.8