Hotspot

petA-psbJ

LSC · 62109-63107 · 999 bp

Back to Farsetia

Pi 0.00901

MarkerSeek score 63.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 9
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	1193-1250 bp	1.0	71.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	1194-1251 bp	1.0	70.8
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1190-1247 bp	1.0	70.4
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.2	57.8	1191-1248 bp	1.0	69.7
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1188-1245 bp	1.0	69.0