Hotspot

rbcL-accD

LSC · 55823-56535 · 713 bp

Back to Farsetia

Pi 0.00847
MarkerSeek score 49.50
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
713
variable_sites
6
pi
0.00847
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AATGAGGGACGTGATCTTGC ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1079-1088 bp 1.0 80.5
rbcL-accD_p2 TTGAATGCTACTGCGGGTAC ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1655-1664 bp 1.0 80.3
rbcL-accD_p3 TGCAATGCACGCTGTTATTG ATTCCGAATGTCCTTGGAGC 45.0 50.0 58.0 58.0 1488-1497 bp 1.0 80.3
rbcL-accD_p4 TCATGGTATGCACTTCCGTG ATTCCGAATGTCCTTGGAGC 50.0 50.0 58.0 58.0 1455-1464 bp 1.0 80.3
rbcL-accD_p5 CTTAACAGGGGGATTCACCG ATTCCGAATGTCCTTGGAGC 55.0 50.0 58.0 58.0 1566-1575 bp 1.0 80.2