Hotspot

rpoB-trnC-UGC

LSC · 26357-27511 · 1155 bp

Back to Farsetia

Pi 0.00929

MarkerSeek score 62.40

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 10
pi: 0.00929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1164-1243 bp	1.0	82.8
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1146-1225 bp	1.0	80.7
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	55.0	50.0	57.9	58.4	1188-1267 bp	1.0	80.1
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1165-1244 bp	1.0	76.8
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1186-1265 bp	1.0	75.4