Hotspot

rpoC2-rpoC1

LSC · 20063-20278 · 216 bp

Back to Farsetia

Pi 0.0165

MarkerSeek score 63.50

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 3
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TGAAATACCAGATTGGCCCG	ATCGAGAAATCGAGGAAGCT	50.0	45.0	57.9	56.4	270-304 bp	1.0	72.0
rpoC2-rpoC1_p2	AATACCAGATTGGCCCGTTC	ATCGAGAAATCGAGGAAGCT	50.0	45.0	57.9	56.4	267-301 bp	1.0	72.0
rpoC2-rpoC1_p3	GAAATACCAGATTGGCCCGT	ATCGAGAAATCGAGGAAGCT	50.0	45.0	57.9	56.4	269-303 bp	1.0	72.0
rpoC2-rpoC1_p4	TGCCATCCCGAAGTGATCTA	ATCGAGAAATCGAGGAAGCT	50.0	45.0	58.2	56.4	340-374 bp	1.0	70.5
rpoC2-rpoC1_p5	AAATACCAGATTGGCCCGTT	ATCGAGAAATCGAGGAAGCT	45.0	45.0	57.5	56.4	268-302 bp	1.0	68.2