Hotspot

trnS-AGC-trnG-GGA

LSC · 7915-8602 · 688 bp

Back to Farsetia

Pi 0.0135

MarkerSeek score 62.70

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 9
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTT	50.0	45.0	57.8	56.5	781-796 bp	1.0	69.2
trnS-AGC-trnG-GGA_p2	CCAACGCTTTAGTCCACTCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	57.8	56.5	780-795 bp	1.0	69.2
trnS-AGC-trnG-GGA_p3	AACGCTTTAGTCCACTCAGC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.2	56.5	779-794 bp	1.0	68.9
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.2	56.5	778-793 bp	1.0	68.9
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTTT	50.0	42.9	57.8	57.2	781-796 bp	1.0	67.3