Hotspot

rps16-trnQ-CAA

LSC · 6142-6620 · 479 bp

Back to Farsetia

Pi 0.0189
MarkerSeek score 67.70
Primer available yes
Label rps16-trnQ-CAA

Feature Metadata

label_name
rps16-trnQ-CAA
feature_type
igs
region
LSC
length_bp
479
variable_sites
9
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-CAA_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 552-560 bp 1.0 80.0
rps16-trnQ-CAA_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 562-570 bp 1.0 79.2
rps16-trnQ-CAA_p3 CCACAGCAGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 578 bp 0.5 56.1
rps16-trnQ-CAA_p4 TCCACAGCAGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 579 bp 0.5 56.0
rps16-trnQ-CAA_p5 ATCCACAGCAGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 580 bp 0.5 54.3