Hotspot

trnK-AAA-rps16

LSC · 4342-4974 · 633 bp

Back to Farsetia

Pi 0.00992
MarkerSeek score 62.90
Primer available yes
Label trnK-AAA-rps16

Feature Metadata

label_name
trnK-AAA-rps16
feature_type
igs
region
LSC
length_bp
633
variable_sites
6
pi
0.00992
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-rps16_p1 AAAGCCGAGTACTCTACCGT AGGCAGGGATTTTTACGGAA 50.0 45.0 58.2 57.1 717-741 bp 1.0 75.3
trnK-AAA-rps16_p2 AAGCCGAGTACTCTACCGTT AAGGCAGGGATTTTTACGGA 50.0 45.0 58.2 57.1 717-741 bp 1.0 75.3
trnK-AAA-rps16_p3 AAGCCGAGTACTCTACCGTT AGGCAGGGATTTTTACGGAA 50.0 45.0 58.2 57.1 716-740 bp 1.0 75.3
trnK-AAA-rps16_p4 AACTCTACCGCAGATCTGGA AAGGCAGGGATTTTTACGGA 50.0 45.0 57.9 57.1 786-810 bp 1.0 75.1
trnK-AAA-rps16_p5 AACTCTACCGCAGATCTGGA AGGCAGGGATTTTTACGGAA 50.0 45.0 57.9 57.1 785-809 bp 1.0 75.1