Hotspot

trnH-CAC-psbA

LSC · 76-361 · 286 bp

Back to Farsetia

Pi 0.0427

MarkerSeek score 78.70

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 12
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	TGTTGAGGCTCCATCTACCA	50.0	50.0	57.9	58.0	337-342 bp	1.0	82.5
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	339-344 bp	1.0	82.5
trnH-CAC-psbA_p3	ACTGCCTTAATCCACTTGGC	TGTTGAGGCTCCATCTACCA	50.0	50.0	58.2	58.0	338-343 bp	1.0	82.3
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	354-359 bp	1.0	82.2
trnH-CAC-psbA_p5	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	357-362 bp	1.0	82.1