Hotspot

petB

LSC · 73791-75274 · 1484 bp

Back to Fargesia

Pi 0.000303

MarkerSeek score 49.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1484
variable_sites: 4
pi: 0.000303
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1570-1591 bp	1.0	68.4
petB_p2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1570-1591 bp	1.0	68.0
petB_p3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1576-1597 bp	1.0	67.6
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1575-1596 bp	1.0	66.2
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1576-1597 bp	1.0	66.2