Hotspot

matK

LSC · 1696-3231 · 1536 bp

Back to Fargesia

Pi 0.00086

MarkerSeek score 47.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 11
pi: 0.00086
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGGGAACATTTCAGAAGAACA	ATGCAACACCCTGTTTTGAC	38.1	45.0	55.0	57.1	1725 bp	1.0	41.8
matK_p2	AGGGAACATTTCAGAAGAACA	AATGCAACACCCTGTTTTGAC	38.1	42.9	55.0	57.8	1726 bp	1.0	40.6
matK_p3	AGGGAACATTTCAGAAGAACA	GCAACACCCTGTTTTGACCA	38.1	50.0	55.0	59.2	1723 bp	1.0	40.6
matK_p4	AGGGAACATTTCAGAAGAACA	TGCAACACCCTGTTTTGACC	38.1	50.0	55.0	59.2	1724 bp	1.0	40.6
matK_p5	AGGGAACATTTCAGAAGAACAT	ATGCAACACCCTGTTTTGAC	36.4	45.0	55.6	57.1	1725 bp	1.0	40.6