Hotspot

rpl14-rpl16

LSC · 80186-80294 · 109 bp

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Pi 0.0111
MarkerSeek score 53.10
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
109
variable_sites
7
pi
0.0111
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AGCAGCTCCTATGACTCGAA TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.2 58.2 246-249 bp 1.0 78.8
rpl14-rpl16_p2 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 55.0 50.0 58.4 58.2 212-215 bp 1.0 77.6
rpl14-rpl16_p3 TAGCAGCTCCTATGACTCGA TAGCTGCCAGTAAAATGCCC 50.0 50.0 57.4 58.2 247-250 bp 1.0 75.6
rpl14-rpl16_p4 AGCAGCTCCTATGACTCGAA GCCAGTAAAATGCCCATACG 50.0 50.0 58.2 57.2 241-244 bp 1.0 74.6
rpl14-rpl16_p5 AGCAGCTCCTATGACTCGAA TGAAATGGGCGGAGTATCTG 50.0 50.0 58.2 57.4 293-296 bp 0.944444 72.3