Hotspot

trnF-GAA-ndhJ

LSC · 50201-50784 · 584 bp

Back to Fargesia

Pi 0.00232

MarkerSeek score 47.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 8
pi: 0.00232
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	688-704 bp	1.0	80.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	678-694 bp	1.0	79.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	719-735 bp	1.0	78.1
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	691-707 bp	1.0	77.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	687-703 bp	1.0	76.5