Hotspot

rps16-trnQ-UUG

LSC · 5608-7140 · 1533 bp

Back to Fargesia

Pi 0.000993

MarkerSeek score 54.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1533
variable_sites: 12
pi: 0.000993
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1546-1620 bp	1.0	82.5
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1557-1631 bp	1.0	82.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1550-1624 bp	1.0	81.4
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1561-1635 bp	1.0	80.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	45.0	45.0	58.1	57.7	1606-1680 bp	1.0	80.6