Hotspot

ndhA

SSC · 129007-131248 · 2242 bp

Back to Fallopia

Pi 0.0268

MarkerSeek score 63.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2242
variable_sites: 114
pi: 0.0268
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACGATTCGCATGGGATAAGG	CCCAAAGGGCGAATTAGGAA	50.0	50.0	57.8	57.9	2620-2678 bp	1.0	82.8
ndhA_p2	CGATTCGCATGGGATAAGGT	CCCAAAGGGCGAATTAGGAA	50.0	50.0	57.8	57.9	2619-2677 bp	1.0	82.8
ndhA_p3	ACGATTCGCATGGGATAAGG	AAATTGGGGGTTGTCAGGAC	50.0	50.0	57.8	58.0	2989 bp	0.25	48.6
ndhA_p4	CGATTCGCATGGGATAAGGT	AAATTGGGGGTTGTCAGGAC	50.0	50.0	57.8	58.0	2988 bp	0.25	48.6
ndhA_p5	AATGTACCTAGCCGCTCGTA	AAATTGGGGGTTGTCAGGAC	50.0	50.0	58.3	58.0	2950 bp	0.25	47.4