Hotspot

rpl32-trnL-UAG

SSC · 122697-123441 · 745 bp

Back to Fallopia

Pi 0.0499

MarkerSeek score 82.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 745
variable_sites: 72
pi: 0.0499
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAAAGGGGAGCCTCTCA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.3	58.1	1678 bp	0.25	47.3
rpl32-trnL-UAG_p2	AGAAAAAGGGGAGCCTCTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	1156 bp	0.25	47.0
rpl32-trnL-UAG_p3	AGAAAAAGGGGAGCCTCTCA	TGGGTAAAAATAGCACCGGG	50.0	50.0	58.3	57.9	1663 bp	0.25	46.9
rpl32-trnL-UAG_p4	AGAAAAAGGGGAGCCTCTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.3	57.8	1149 bp	0.25	46.5
rpl32-trnL-UAG_p5	AGAAAAAGGGGAGCCTCTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.3	57.8	1154 bp	0.25	46.4