Hotspot

ndhF-rpl32

SSC · 121440-122522 · 1083 bp

Back to Fallopia

Pi 0.048
MarkerSeek score 80.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1083
variable_sites
87
pi
0.048
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.89
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCTGCTCCGATTAACAAAGGA TGGAAATTGAAGTACGCTTT 47.6 35.0 58.0 53.4 1085-1191 bp 1.0 50.2
ndhF-rpl32_p2 CCTGCTCCGATTAACAAAGGA TTGGAAATTGAAGTACGCTT 47.6 35.0 58.0 53.4 1086-1192 bp 1.0 50.2
ndhF-rpl32_p3 CCTGCTCCGATTAACAAAGGA TTTGGAAATTGAAGTACGCT 47.6 35.0 58.0 53.4 1087-1193 bp 1.0 50.2
ndhF-rpl32_p4 CCTGCTCCGATTAACAAAGGA TGGAAATTGAAGTACGCTTTT 47.6 33.3 58.0 54.3 1085-1191 bp 1.0 50.2
ndhF-rpl32_p5 CCTGCTCCGATTAACAAAGGA TTGGAAATTGAAGTACGCTTT 47.6 33.3 58.0 54.3 1086-1192 bp 1.0 50.2