Hotspot

rps18-rpl20

LSC · 72425-72649 · 225 bp

Back to Fallopia

Pi 0.0751

MarkerSeek score 77.50

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 28
pi: 0.0751
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TTCCTTAAACCTAAGCGGCC	ACTCGGATAAACGCAGCAAT	50.0	45.0	57.9	58.0	709-726 bp	1.0	86.1
rps18-rpl20_p2	CCTTAAACCTAAGCGGCCTT	ACTCGGATAAACGCAGCAAT	50.0	45.0	57.9	58.0	707-724 bp	1.0	86.1
rps18-rpl20_p3	TTCCTTAAACCTAAGCGGCC	CTTTCGAGGGGCTCATTCAA	50.0	50.0	57.9	57.9	827-844 bp	1.0	84.6
rps18-rpl20_p4	TTCCTTAAACCTAAGCGGCC	TTGGAAAGAAGCACCCGAAA	50.0	45.0	57.9	57.9	541-547 bp	0.5	64.7
rps18-rpl20_p5	CCTTAAACCTAAGCGGCCTT	TTGGAAAGAAGCACCCGAAA	50.0	45.0	57.9	57.9	539-545 bp	0.5	64.7