Hotspot

psaJ-rpl33

LSC · 70876-71416 · 541 bp

Back to Fallopia

Pi 0.0569

MarkerSeek score 80.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 55
pi: 0.0569
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGTGGTTCGGATCTTTAGCG	GCTTGGAACAGTAGGGACAG	50.0	55.0	58.0	57.9	666-799 bp	1.0	89.8
psaJ-rpl33_p2	TGTGGTTCGGATCTTTAGCG	ACTCGTGCTTTAAAAGGCGA	50.0	45.0	58.0	58.1	736-869 bp	1.0	89.2
psaJ-rpl33_p3	TGTGGTTCGGATCTTTAGCG	GGCCGCTTAGGTTTAAGGAA	50.0	50.0	58.0	57.9	1210-1361 bp	1.0	88.7
psaJ-rpl33_p4	TGTGGTTCGGATCTTTAGCG	AAGGCCGCTTAGGTTTAAGG	50.0	50.0	58.0	57.9	1212-1363 bp	1.0	88.6
psaJ-rpl33_p5	AGGCTTTTAGGGCAGTAGGA	GCTTGGAACAGTAGGGACAG	50.0	55.0	58.0	57.9	1080 bp	0.25	48.9