Hotspot

petG-trnW-CCA

LSC · 69798-69904 · 107 bp

Back to Fallopia

Pi 0.0685

MarkerSeek score 76.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 107
variable_sites: 11
pi: 0.0685
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ATAGGCGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.7	58.0	189-190 bp	1.0	81.8
petG-trnW-CCA_p2	ATAGGCGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.7	57.9	175-176 bp	1.0	81.8
petG-trnW-CCA_p3	ATAGGCGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.7	58.0	211-212 bp	1.0	81.5
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.4	58.0	183-184 bp	1.0	79.9
petG-trnW-CCA_p5	GTGGTGATCAGTTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.4	58.0	205-206 bp	1.0	79.6