Hotspot

ycf4-cemA

LSC · 63695-64584 · 890 bp

Back to Fallopia

Pi 0.0474

MarkerSeek score 82.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 77
pi: 0.0474
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TATTTCTTGCGCGTACCGAT	TCCACCAGGGTAAACAGACT	45.0	50.0	57.8	57.9	960-990 bp	1.0	87.7
ycf4-cemA_p2	TATTTCTTGCGCGTACCGAT	CCACCAGGGTAAACAGACTG	45.0	55.0	57.8	57.8	959-989 bp	1.0	87.1
ycf4-cemA_p3	TATTTCTTGCGCGTACCGAT	AGAAAGCTCCACCAGGGTAA	45.0	50.0	57.8	58.3	967-997 bp	1.0	86.5
ycf4-cemA_p4	TATTTCTTGCGCGTACCGAT	AAAGCTCCACCAGGGTAAAC	45.0	50.0	57.8	57.7	965-995 bp	1.0	86.2
ycf4-cemA_p5	TATTTCTTGCGCGTACCGAT	CTCCACCAGGGTAAACAGAC	45.0	55.0	57.8	57.5	961-991 bp	1.0	84.8