Hotspot

trnV-UAC-trnM-CAU

LSC · 55237-55412 · 176 bp

Back to Fallopia

Pi 0.038

MarkerSeek score 75.60

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 13
pi: 0.038
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	ACCAACTGAGCTATAGCCCT	TTGAACCAATGACTCCTGCC	50.0	50.0	57.8	58.1	249-254 bp	1.0	83.6
trnV-UAC-trnM-CAU_p2	CCTTGAAAAACATTGGCGCA	TTGAACCAATGACTCCTGCC	45.0	50.0	58.1	58.1	288-293 bp	1.0	83.2
trnV-UAC-trnM-CAU_p3	ACCAACTGAGCTATAGCCCT	CTCCTGCCGTATGAAAGCAA	50.0	50.0	57.8	58.3	237-242 bp	1.0	82.5
trnV-UAC-trnM-CAU_p4	CCTTGAAAAACATTGGCGCA	CTCCTGCCGTATGAAAGCAA	45.0	50.0	58.1	58.3	276-281 bp	1.0	82.0
trnV-UAC-trnM-CAU_p5	CCTTGAAAAACATTGGCGCA	CCAATGACTCCTGCCGTATG	45.0	55.0	58.1	58.4	283-288 bp	1.0	80.7